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Signal Transduction Signaling Pathway Lipid Signaling PLA

Recombinant Mouse PLA2G12B protein (His tag) (ab276901)

Recombinant Mouse PLA2G12B protein (His tag) (ab276901)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Mouse PLA2G12B protein (His tag)
    See all PLA2G12B proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    Q99P27
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MKLLCGFFLL WLGLVGNLAQ SDPSPKEEES YSDWGLRQLR GSFESVNSYV DSFMELLGGK NGVCQYRCR YGKAPMPRPG YKAQEPNGCS SYFLGIKVPG SMDLGIPAMT KCCNQLDVCY DTCGANKYR CDAKFRWCLH SICSDLKRTL GFVSNVEAAC DSLADTVFNT VWTLGCRPFM NSQRAACIC AEEEKEEL
    • Predicted molecular weight

      21 kDa including tags
    • Molecular weight information

      The secreted recombinant mouse PLA2G12B comprises 187 amino acids and has a calculated molecular mass of 21 kDa. The apparent molecular mass of the recombinant protein is approximately 24 kDa in SDS-PAGE under reducing conditions.
    • Amino acids

      1 to 195
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Predicted N-terminus: Gln 20.
  • Specifications

    Our Abpromise guarantee covers the use of ab276901 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 5
      Constituents: 0.16% Sodium acetate, 0.58% Sodium chloride

    • Reconstitution
      This information is lot specific. Please contact our technical Support team for details.

    General Info

    • Alternative names

      • 2010002E04Rik
      • FKSG71
      • Group XIIB phospholipase A2
      • Group XIII secreted phospholipase A2
      • GXIIB
      • GXIIIsPLA2
      • MGC138151
      • Phospholipase A2, group XIIB
      • PLA2G12B
      • PLA2G13
      see all
    • Relevance

      Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]).
    • Cellular localization

      Secreted

Images

  • SDS-PAGE - Recombinant Mouse PLA2G12B protein (ab276901)
    SDS-PAGE - Recombinant Mouse PLA2G12B protein (ab276901)
    SDS-PAGE analysis of ab276901

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab276901? Please let us know so that we can cite the reference in this datasheet.

    ab276901 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • 2010002E04Rik
    • FKSG71
    • Group XIIB phospholipase A2

    Images

    • SDS-PAGE - Recombinant Mouse PLA2G12B protein (ab276901)
      SDS-PAGE - Recombinant Mouse PLA2G12B protein (ab276901)
      SDS-PAGE analysis of ab276901

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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