Recombinant Mouse PLA2G12B protein (His tag) (ab276901)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Mouse PLA2G12B protein (His tag)
See all PLA2G12B proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Full length proteinAnimal free
NoNature
Recombinant-
Species
Mouse -
Sequence
MKLLCGFFLL WLGLVGNLAQ SDPSPKEEES YSDWGLRQLR GSFESVNSYV DSFMELLGGK NGVCQYRCR YGKAPMPRPG YKAQEPNGCS SYFLGIKVPG SMDLGIPAMT KCCNQLDVCY DTCGANKYR CDAKFRWCLH SICSDLKRTL GFVSNVEAAC DSLADTVFNT VWTLGCRPFM NSQRAACIC AEEEKEEL -
Predicted molecular weight
21 kDa including tags -
Molecular weight information
The secreted recombinant mouse PLA2G12B comprises 187 amino acids and has a calculated molecular mass of 21 kDa. The apparent molecular mass of the recombinant protein is approximately 24 kDa in SDS-PAGE under reducing conditions. -
Amino acids
1 to 195 -
Tags
His tag C-Terminus -
Additional sequence information
Predicted N-terminus: Gln 20.
Specifications
Our Abpromise guarantee covers the use of ab276901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 5
Constituents: 0.16% Sodium acetate, 0.58% Sodium chloride -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- 2010002E04Rik
- FKSG71
- Group XIIB phospholipase A2
see all -
Relevance
Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]). -
Cellular localization
Secreted
Images
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SDS-PAGE - Recombinant Mouse PLA2G12B protein (ab276901)SDS-PAGE analysis of ab276901
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab276901 has not yet been referenced specifically in any publications.
Preparation and Storage
- 2010002E04Rik
- FKSG71
- Group XIIB phospholipase A2
Images
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SDS-PAGE - Recombinant Mouse PLA2G12B protein (ab276901)SDS-PAGE analysis of ab276901