Human BRE (BRCC45) knockout HeLa cell lysate (ab257861)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260372 - Human BRE knockout HeLa cell lysate 1 x 100µg ab255552 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human BRE knockout HeLa cell lysate (ab257861)
Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: BRE knockout HeLa cell lysate (20 µg)
Lane 3: SH-SY5Y cell lysate (20 µg)
Lane 4: Daudi cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab177960 observed at 44 kDa. Red - loading control ab7291 observed at 50 kDa.
ab177960 Recombinant Anti-BRE antibody [EPR11858] was shown to specifically react with BRE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264928 (knockout cell lysate ab257861) was used. Wild-type and BRE knockout samples were subjected to SDS-PAGE. ab177960 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human BRE knockout HeLa cell lysate (ab257861)Homozygous: Insertion of the selection cassette in exon 3