Urinary Prostacyclin ELISA kit (ab133032)
Key features and details
- Sensitivity: 6.58 pg/ml
- Range: 7.81 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Tissue Culture Media, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Urinary Prostacyclin ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low 24 23pg/ml 9.1% Medium 24 66pg/ml 3.4% High 24 200pg/ml 2.9% Inter-assay Sample n Mean SD CV% Low 8 23pg/ml 9.6% Medium 8 72pg/ml 5.4% High 8 223pg/ml 3.4% -
Sample type
Cell culture supernatant, Urine, Tissue Culture Media -
Assay type
Competitive -
Sensitivity
6.58 pg/ml -
Range
7.81 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 101.9 % - % Tissue Culture Media 109.2 % - % -
Assay time
3h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species
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Product overview
Abcam’s Human Urinary Prostacyclin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostacyclin breakdown products in urine. The kit measures the two major breakdown products of Prostacyclin in urine, 6-keto-Prostaglandin F1 alpha (6k-PGF1 alpha) and 2,3-dinor-6-keto-Prostaglandin F1 alpha (2,3d-6k-PGF1 alpha).
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Urinary Prostacyclin antigen and a polyclonal sheep antibody specific to Urinary Prostacyclin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Urinary Prostacyclin captured in the plate.
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Notes
Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6k-PGF1α and 2,3d-6k- PGF1α. 6-keto- PGF1α is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2. Methods to measure PGI2 in urine typically involve HPLC, gas chromatography/mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6k- PGF1α.
Cross Reactivity
The cross reactivity for a number of related eicosanoid compounds was determined.
Compound Cross Reactivity 2,3-dinor-6-keto-PGF1α 100% 6-keto-PGF1α 89.14% PGF1α 13.21% PGF2α 4.99% 13,14 dihydro-PGF1α 2.55% 15-keto-PGF2α 0.32% PGE1 0.08% PGD2 0.03% 6,15-diketo-13,14 dihydro-PGF1α 0.03% PGB1 0.01% PGE2 0.01% PGA2 0.01% TXB2 0.01% -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit Human Urinary Prostacyclin Standard 1 x 500µl Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml Urinary Prostacyclin Alkaline Phosphatase Conjugate 1 x 5ml Urinary Prostacyclin Antibody 1 x 5ml -
Research areas
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Alternative names
- 2,3-dinor-6-keto-Prostaglandin F1 alpha
- 2,3d-6k- PGF1 alpha
- 6-keto-Prostaglandin F1 alpha
see all
Images
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Typical Standard Curve
Representative Standard Curve using ab133032