Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MYL9 antibody [EPR13012(2)] (ab191393)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13012(2)] to MYL9
Suitable for: WB, IHC-P, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-MYL9 antibody [EPR13012(2)]
See all MYL9 primary antibodies
Description

Rabbit monoclonal [EPR13012(2)] to MYL9
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Rat Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, Hu colon, C6, PC12 and NIH 3T3 cell lysates; Human stomach, colon cancer and uterus lysates; Rat stomach lysate. IHC-P: Human and rat colon tissues. IP: Human stomach lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13012(2)
Isotype

IgG
Research areas

Western blot - Anti-MYL9 antibody [EPR13012(2)] (ab191393)

All lanes : Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MYL9 knockout HeLa cell lysate
Lane 3 : Human colon cell lysate
Lane 4 : Daudi cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 20 kDa

Lanes 1-4: Merged signal (red and green). Green - ab191393 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.

ab191393 Anti-MYL9 antibody [EPR13012(2)] was shown to specifically react with MYL9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266036 (knockout cell lysate ab256999) was used. Wild-type and MYL9 knockout samples were subjected to SDS-PAGE. ab191393 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 [EPR13012(2)] antibody (ab191393)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MYL9 with ab191393 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)

All lanes : Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/1000 dilution

Lane 1 : Human stomach lysate
Lane 2 : Human colon cancer lysate
Lane 3 : Human uterus lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa
Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)

All lanes : Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa
Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)

Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/5000 dilution + rat stomach lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 [EPR13012(2)] antibody (ab191393)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MYL9 with ab191393 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-MYL9 [EPR13012(2)] antibody (ab191393)

Western blot analysis of immunoprecipitation pellet from Human stomach lysate immunoprecipitated using ab191393 at 1/40 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-MYL9 antibody [EPR13012(2)] (ab191393)

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