Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MYL9 antibody [EPR13012(2)] (ab191393)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13012(2)] to MYL9
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MYL9 antibody [EPR13012(2)]
    See all MYL9 primary antibodies

  • Description

    Rabbit monoclonal [EPR13012(2)] to MYL9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Hu colon, C6, PC12 and NIH 3T3 cell lysates; Human stomach, colon cancer and uterus lysates; Rat stomach lysate. IHC-P: Human and rat colon tissues. IP: Human stomach lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13012(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MYL9 antibody [EPR13012(2)] (ab191393)
    Western blot - Anti-MYL9 antibody [EPR13012(2)] (ab191393)
    All lanes : Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : MYL9 knockout HeLa cell lysate
    Lane 3 : Human colon cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 20 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab191393 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab191393 Anti-MYL9 antibody [EPR13012(2)] was shown to specifically react with MYL9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266036 (knockout cell lysate ab256999) was used. Wild-type and MYL9 knockout samples were subjected to SDS-PAGE. ab191393 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 [EPR13012(2)] antibody (ab191393)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 [EPR13012(2)] antibody (ab191393)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MYL9 with ab191393 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)
    Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)
    All lanes : Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/1000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : Human colon cancer lysate
    Lane 3 : Human uterus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa

  • Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)
    Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)
    All lanes : Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa

  • Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)
    Western blot - Anti-MYL9 [EPR13012(2)] antibody (ab191393)
    Anti-MYL9 antibody [EPR13012(2)] (ab191393) at 1/5000 dilution + rat stomach lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 [EPR13012(2)] antibody (ab191393)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 [EPR13012(2)] antibody (ab191393)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MYL9 with ab191393 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-MYL9 [EPR13012(2)] antibody (ab191393)
    Immunoprecipitation - Anti-MYL9 [EPR13012(2)] antibody (ab191393)

    Western blot analysis of immunoprecipitation pellet from Human stomach lysate immunoprecipitated using ab191393 at 1/40 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-MYL9 antibody [EPR13012(2)] (ab191393)
    Anti-MYL9 antibody [EPR13012(2)] (ab191393)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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