Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13013(2)(B)] to MYL9
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-MYL9 antibody [EPR13013(2)(B)]
See all MYL9 primary antibodies
Description

Rabbit monoclonal [EPR13013(2)(B)] to MYL9
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa and human colon cell lysate; Human stomach, colon and uterus lysates; Mouse muscle lysate. IHC-P: Human colon tissue; Mouse stomach tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13013(2)(B)
Isotype

IgG
Research areas

Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

All lanes : Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MYL9 knockout HeLa cell lysate
Lane 3 : Human colon cell lysate
Lane 4 : Daudi cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 20 kDa

Lanes 1-4: Merged signal (red and green). Green - ab187152 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.

ab187152 Anti-MYL9 antibody [EPR13013(2)(B)] was shown to specifically react with MYL9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266036 (knockout cell lysate ab256999) was used. Wild-type and MYL9 knockout samples were subjected to SDS-PAGE. ab187152 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling MYL9 with ab187152 at 1/500 dilution. Anti-rabbit IgG HRP was used as a secondary antibody and slides were counterstained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MYL9 with ab187152 at 1/500 dilution. Anti-rabbit IgG HRP was used as a secondary antibody and slides were counterstained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152) at 1/1000 dilution + Mouse muscle lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa
Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

All lanes : Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152) at 1/5000 dilution

Lane 1 : Human stomach lysate
Lane 2 : Human colon lysate
Lane 3 : Human uterus lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 20 kDa
Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

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