Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11350] to WDR5 - C-terminal
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-WDR5 antibody [EPR11350] - C-terminal
    See all WDR5 primary antibodies

  • Description

    Rabbit monoclonal [EPR11350] to WDR5 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human WDR5 aa 300 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: P61964

  • Positive control

    • HeLa and Saos-2 cell lysates; Human breast and Human thyroid gland tissues; HeLa cells. RAW264.7 and Mouse spleen, rat spleen lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
    Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
    All lanes : Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution

    Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 36 kDa

  • Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
    Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
    All lanes : Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Saos-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 36 kDa
    Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.

  • Flow Cytometry - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
    Flow Cytometry - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)

    Flow Cytometrical analysis of permeabilized HeLa cells labeling WDR5 with ab178410 at 1/10 (red) compared to a nonspecific negative control antibody (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling WDR5 with ab178410 at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)

    Immunohistochemical analysis of paraffin embedded Human breast tissue labeling WDR5 with ab178410 at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
    Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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