Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WDR5 antibody [2C2] (ab56919)

Key features and details

  • Mouse monoclonal [2C2] to WDR5
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-WDR5 antibody [2C2]
    See all WDR5 primary antibodies

  • Description

    Mouse monoclonal [2C2] to WDR5

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment, corresponding to amino acids 16-121 of Human WDR5

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-WDR5 antibody [2C2] (ab56919)
    Western blot - Anti-WDR5 antibody [2C2] (ab56919)

    WDR5 antibody (ab56919) at 1μg/lane + Hs 181 S cell lysate at 25μg/lane.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-WDR5 antibody [2C2] (ab56919)
    Flow Cytometry - Anti-WDR5 antibody [2C2] (ab56919)

    Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (ab56919)
    Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (ab56919)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR5 (green) using ab56919 at 10 µg/ml in ICC/IF. 

    This image was generated using the ascites version of the product.

  • Western blot - Anti-WDR5 antibody [2C2] (ab56919)
    Western blot - Anti-WDR5 antibody [2C2] (ab56919)
    Anti-WDR5 antibody [2C2] (ab56919) at 1 µg/ml + Hs 181.Tes cell lysate

    Predicted band size: 36 kDa



    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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