Anti-HIF1AN/FIH-1 antibody (ab233141)
Key features and details
- Rabbit polyclonal to HIF1AN/FIH-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-HIF1AN/FIH-1 antibody
See all HIF1AN/FIH-1 primary antibodies -
Description
Rabbit polyclonal to HIF1AN/FIH-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse
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Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human HIF1AN/FIH-1 aa 1 to the C-terminus. Expressed in E.coli. N-terminal tags.
Database link: Q9NWT6 -
Positive control
- IHC-P: Human prostate, stomach cancer, stomach and skin cancer tissues. ICC/IF: HeLa cells. WB: HeLa and HEK-293T cell lysates; Pig kidney and brain lysates; Recombinant human HIF1AN/FIH-1 protein.
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General notes
Previously labelled as HIF1AN.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab233141 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HIF1AN/FIH-1 antibody (ab233141)All lanes : Anti-HIF1AN/FIH-1 antibody (ab233141) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Pig kidney lysate
Lane 4 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 40 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN / FIH-1 antibody (ab233141)Paraffin-embedded human prostate tissue stained for HIF1AN/FIH-1 using ab233141 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN / FIH-1 antibody (ab233141)Paraffin-embedded human stomach cancer tissue stained for HIF1AN/FIH-1 using ab233141 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody (ab233141)Paraffin-embedded human stomach tissue stained for HIF1AN/FIH-1 using ab233141 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody (ab233141)Paraffin-embedded human skin cancer tissue stained for HIF1AN/FIH-1 using ab233141 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-HIF1AN/FIH-1 antibody (ab233141)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HIF1AN/FIH-1 (green) using ab233141 at 20 µg/ml in ICC/IF, followed by FITC staining.
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Western blot - Anti-HIF1AN/FIH-1 antibody (ab233141)Anti-HIF1AN/FIH-1 antibody (ab233141) at 2 µg/ml + Recombinant human HIF1AN/FIH-1 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 40 kDa
