Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3658] to HIF1AN/FIH-1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-HIF1AN/FIH-1 antibody [EPR3658]
    See all HIF1AN/FIH-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3658] to HIF1AN/FIH-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human HIF1AN/FIH-1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Jurkat, 293T and A375 cell lysates; Human breast carcinoma and brain tissue; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as HIF1AN.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)
    Western blot - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)
    All lanes : Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A375 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

    ab92498 at 1/250 dilution staining HIF1AN/FIH-1 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

    ab92498 at 1/250 dilution staining HIF1AN/FIH-1 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

    ab92498 at 1/100 dilution staining HIF1AN/FIH-1 in HeLa cells by Immunofluorescence.

  • Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)
    Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

© 2021 Astana Biomed Group LLP. All rights reserved.