Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3658] to HIF1AN/FIH-1
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-HIF1AN/FIH-1 antibody [EPR3658]
See all HIF1AN/FIH-1 primary antibodies
Description

Rabbit monoclonal [EPR3658] to HIF1AN/FIH-1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human HIF1AN/FIH-1 aa 1-100. The exact sequence is proprietary.
Positive control
- Jurkat, 293T and A375 cell lysates; Human breast carcinoma and brain tissue; HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Previously labelled as HIF1AN.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3658
Isotype

IgG
Research areas

Western blot - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

All lanes : Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498) at 1/5000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A375 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 40 kDa
Observed band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

ab92498 at 1/250 dilution staining HIF1AN/FIH-1 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

ab92498 at 1/250 dilution staining HIF1AN/FIH-1 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

ab92498 at 1/100 dilution staining HIF1AN/FIH-1 in HeLa cells by Immunofluorescence.
Anti-HIF1AN/FIH-1 antibody [EPR3658] (ab92498)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com