Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13813] to NSD3 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NSD3 antibody [EPR13813] - BSA and Azide free
    See all NSD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13813] to NSD3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250203 is the carrier-free version of ab180500. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250203 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    Western blot - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (unpurified)

    Lane 1 : Hela lysate
    Lane 2 : A673 lysate
    Lane 3 : MCF7 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 162 kDa



    This data was developed using ab180500, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    Flow Cytometry - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)

    This data was developed using ab180500, the same antibody clone in a different buffer formulation.

    Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab180500 at a dilution of 1 in 70 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of primary and secondary antibody were used as a negative control (blue line).
  • Western blot - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    Western blot - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified) + Rat kidney tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 162 kDa
    Observed band size: 162 kDa



    This data was developed using ab180500, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    Western blot - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/10000 dilution (purified)

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 162 kDa
    Observed band size: 162 kDa



    This data was developed using ab180500, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    Western blot - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 162 kDa
    Observed band size: 162 kDa



    This data was developed using ab180500, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    Flow Cytometry - Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    This data was developed using ab180500, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of HeLa cells labeling NSD3 with unpurified ab180500 at 1/75 (green) compared to a negative control antibody (red).
  • Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)
    Anti-NSD3 antibody [EPR13813] - BSA and Azide free (ab250203)

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