Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7978] to HEXB - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-HEXB antibody [EPR7978] - BSA and Azide free
    See all HEXB primary antibodies

  • Description

    Rabbit monoclonal [EPR7978] to HEXB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248929 is the carrier-free version of ab140649. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248929 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)
    Western blot - Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)

    This data was developed using ab140649, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: HEXB knockout HAP1 whole cell lysate (20 µg)

    Lane 3: Jurkat whole cell lysate (20 µg)

    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab140649 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab140649 was shown to specifically react with HEXB when HEXB knockout samples were used. Wild-type and HEXB knockout samples were subjected to SDS-PAGE. ab140649 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. This image was generated using the unpurified version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)
    This data was developed using ab140649, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling HEXB with Purified ab140649 at 1:500 dilution (4.84 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)
    Western blot - Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)
    All lanes : Anti-HEXB antibody [EPR7978] (ab140649) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa
    Observed band size: 29,63 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab140649, the same antibody clone in a different buffer formulation.

    The double bands caused by proteolytic processing are consistent with what has been described in PMID: 2139028.

  • Western blot - Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)
    Western blot - Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)
    All lanes : Anti-HEXB antibody [EPR7978] (ab140649) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 63 kDa



    This data was developed using ab140649, the same antibody clone in a different buffer formulation.

    This image was generated using the unpurified version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)
    This data was developed using ab140649, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HEXB with ab140649 at a 1/25 dilution. This image was generated using the unpurified version of the product.
  • Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)
    Anti-HEXB antibody [EPR7978] - BSA and Azide free (ab248929)

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