Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14908] to PGK1 + PGK2
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-PGK1 + PGK2 antibody [EPR14908]
See all PGK1 + PGK2 primary antibodies
Description

Rabbit monoclonal [EPR14908] to PGK1 + PGK2
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human testis lysate; Jurkat, HepG2, HeLa, C6, Raw 264.7, PC-12 and NIH 3T3 cell lysates; Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14908
Isotype

IgG
Research areas

Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

All lanes : Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/20000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : Human testis cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa
Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

All lanes : Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/5000 dilution

Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa
Observed band size: 45 kDa
Flow Cytometry - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

Flow Cytometric analysis of 2% paraformaldehyde fixed Jurkat cells labeling PGK1 with ab186742 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com