Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
![Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)](https://www.abcam.com/ps/products/186/ab186742/Images/ab186742-220116-anti-pgk1-pgk2-antibody-epr14908-western-blot.jpg "Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14908] to PGK1 + PGK2
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PGK1 + PGK2 antibody [EPR14908]
See all PGK1 + PGK2 primary antibodies -
Description
Rabbit monoclonal [EPR14908] to PGK1 + PGK2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB RatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human testis lysate; Jurkat, HepG2, HeLa, C6, Raw 264.7, PC-12 and NIH 3T3 cell lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14908 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)All lanes : Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human testis cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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![Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)](https://www.abcam.com/ps/products/186/ab186742/Images/ab186742-220115-anti-pgk1-pgk2-antibody-epr14908-western-blot.jpg)
Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)All lanes : Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/5000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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![Flow Cytometry - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)](https://www.abcam.com/ps/products/186/ab186742/Images/ab186742-220114-anti-pgk1-pgk2-antibody-epr14908-flow-cytometry.jpg)
Flow Cytometry - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)Flow Cytometric analysis of 2% paraformaldehyde fixed Jurkat cells labeling PGK1 with ab186742 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)](https://www.abcam.com/ps/products/186/ab186742/Images/ab186742-4-benefits-of-recombinant-antibodies.png)
Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)