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Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free
    See all Glycogen synthase 1/GYS1 primary antibodies

  • Description

    Rabbit monoclonal [EP817Y] to Glycogen synthase 1/GYS1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa whole cell lysate (ab29545) can be used as a positive control in WB.

  • General notes

    ab231693 is the carrier-free version of ab40810 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab231693 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Glycogen synthase 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)
    Western blot - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)
    All lanes : Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] (ab40810) at 1/10000 dilution

    Lane 1 : Wild-type HeLa lysate
    Lane 2 : Glycogen synthase 1/GYS1 knockout HeLa lysate
    Lane 3 : HEK-293 lysate
    Lane 4 : Daudi lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 81 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab40810).

    Lanes 1-4: Merged signal (red and green). Green - ab40810 observed at 80 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab40810 Recombinant Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] was shown to specifically react with Glycogen synthase 1/GYS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265388 (knockout cell lysate ab257462) was used. Wild-type and Glycogen synthase 1/GYS1 knockout samples were subjected to SDS-PAGE. ab40810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Flow Cytometry - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)
    Flow Cytometry - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)
    Overlay histogram showing HEK293 cells stained with ab40810 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40810, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40810).

  • Immunocytochemistry/ Immunofluorescence - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)
    Immunocytochemistry/ Immunofluorescence - Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)

    ab40810 at a 1:250 dilution staining Hela cells using anti-Glycogen Synthase 1/GYS1 RabMAb.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40810).

  • Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)
    Anti-Glycogen synthase 1/GYS1 antibody [EP817Y] - BSA and Azide free (ab231693)

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