Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP852Y] to Glycogen synthase 1/GYS1 (phospho S641)
Suitable for: Dot blot, WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y]
See all Glycogen synthase 1/GYS1 primary antibodies
Description

Rabbit monoclonal [EP852Y] to Glycogen synthase 1/GYS1 (phospho S641)
Host species

Rabbit
Specificity

Detects Glycogen synthase 1/GYS1 only when phosphorylated on Serine 641.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human Glycogen synthase 1/GYS1. The exact sequence is proprietary.
Positive control
- WB: Mouse muscle and Mouse muscle treated with AP lysates; HeLa and HeLa treated with insulin at 20 ug/mL for 15 minutes whole cell lysates. IHC: Human liver tissue.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as Glycogen synthase 1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP852Y
Isotype

IgG
Research areas

Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)

All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230) at 1/2000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with insulin at 20 ug/mL for 15 minutes. Whole cell lysates
Lane 2 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates.
Lane 3 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates.Then the membrane was incubated with phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 85 kDa
Observed band size: 85 kDa

Exposure time: 1 second

Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)

All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230) at 1/10000 dilution

Lane 1 : Mouse muscle lysate, untreated
Lane 2 : Mouse muscle lysate, treated with AP

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 85 kDa
Observed band size: 85 kDa
Dot Blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)

Dot blot analysis of glycogen synthase 1/GYS1 (phospho S641) phospho peptide (Lane 1) and glycogen synthase 1/GYS1 non-phospho peptide (Lane 2) labeling synthase 1 (phospho S641) with ab81230 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)

Immunohistochemical analysis of Human liver tissue, using 1/100 ab81230.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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