Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479)
Key features and details
- Rabbit polyclonal to Glycogen synthase 1/GYS1 (phospho S640)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody
See all Glycogen synthase 1/GYS1 primary antibodies -
Description
Rabbit polyclonal to Glycogen synthase 1/GYS1 (phospho S640) -
Host species
Rabbit -
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated Ser 640 of human muscle glycogen synthase (GYS1). Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Glycogen synthase 1/GYS1 aa 631-642 conjugated to keyhole limpet haemocyanin.
Sequence: AQGYRYPRPASpVP -
General notes
Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.
This product was previously labelled as Glycogen synthase 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
Primary antibody notes
Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479)
Immunohistochemistry with Anti-Glycogen Synthase antibody Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody(ab2479): 5 µg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
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Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479)All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S640) antibody (ab2479) at 1/1000 dilution
Lanes 1 & 5 : mock treated mouse cardiac myocyte lysate at 12 µl
Lane 2 : insulin (10 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 3 : insulin (100 nM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 4 : insulin (1 mM, 15min) treated mouse cardiac myocyte lysate at 12 µl
Lane 6 : CLA treated (4 nM, 45min) mouse cardiac myocyte lysate at 12 µl
Lane 7 : CLA treated (20 nM, 45min) mouse cardiac myocyte lysate
Lane 8 : CLA treated (100 nM, 45min) mouse cardiac myocyte lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilutionRabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.
Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.
A 4-20% Criterion gel for SDS-PAGE was used.
