Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Tuesday March 16, 2021

Anti-BPGM antibody [EPR15128] (ab186739)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15128] to BPGM
Suitable for: WB, Flow Cyt, IP
Reacts with: Human

Product name

Anti-BPGM antibody [EPR15128]
See all BPGM primary antibodies
Description

Rabbit monoclonal [EPR15128] to BPGM
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human placenta lysate, Human blood lysate; HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15128
Isotype

IgG
Research areas

Western blot - Anti-BPGM antibody [EPR15128] (ab186739)

Anti-BPGM antibody [EPR15128] (ab186739) at 1/10000 dilution + Human blood lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 30 kDa
Observed band size: 30 kDa
Immunoprecipitation - Anti-BPGM antibody [EPR15128] (ab186739)

Western blot analysis on immunoprecipitation from 1) Human blood lysate and 2) PBS, labeling BPGM using 186739 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
Western blot - Anti-BPGM antibody [EPR15128] (ab186739)

Anti-BPGM antibody [EPR15128] (ab186739) at 1/1000 dilution + Human placenta lysate at 20 µg

Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 30 kDa
Observed band size: 30 kDa
Flow Cytometry - Anti-BPGM antibody [EPR15128] (ab186739)

Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling BPGM with ab186739 at a 1/110 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
Anti-BPGM antibody [EPR15128] (ab186739)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com