Anti-BPGM antibody [EPR15128] (ab186739)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15128] to BPGM
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-BPGM antibody [EPR15128]
See all BPGM primary antibodies -
Description
Rabbit monoclonal [EPR15128] to BPGM -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human placenta lysate, Human blood lysate; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15128 -
Isotype
IgG -
Research areas
Images
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Anti-BPGM antibody [EPR15128] (ab186739) at 1/10000 dilution + Human blood lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 30 kDa
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Western blot analysis on immunoprecipitation from 1) Human blood lysate and 2) PBS, labeling BPGM using 186739 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
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Anti-BPGM antibody [EPR15128] (ab186739) at 1/1000 dilution + Human placenta lysate at 20 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 30 kDa
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Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling BPGM with ab186739 at a 1/110 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
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