Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD75 antibody [EPR22054-238] (ab225793)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22054-238] to CD75
Suitable for: Flow Cyt, ICC/IF, IP, WB
Reacts with: Human

Product name

Anti-CD75 antibody [EPR22054-238]
See all CD75 primary antibodies
Description

Rabbit monoclonal [EPR22054-238] to CD75
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Daudi, Raji and THP-1 whole cell lysates. ICC/IF: Daudi cells. Flow Cyt: Daudi cells. IP: Raji whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22054-238
Isotype

IgG
Research areas

Western blot - Anti-CD75 antibody [EPR22054-238] (ab225793)

All lanes : Anti-CD75 antibody [EPR22054-238] (ab225793) at 1/1000 dilution

Lane 1 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : HFF-1 (human skin fibroblast cell line) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 47 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with the literature (PMID 23358684).

Negative control: HFF-1 (PMID: 23358684).
Immunocytochemistry/ Immunofluorescence - Anti-CD75 antibody [EPR22054-238] (ab225793)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi (human Burkitt's lymphoma cell line) and HFF-1 (human skin fibroblast cell line) cells labeling CD75 with ab225793 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining in Daudi cells, likely localized to the Golgi (PMID: 23578204).

Negative control: HFF-1 (human skin fibroblast) [PMID: 23358684].

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-CD75 antibody [EPR22054-238] (ab225793)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HFF-1 (human skin fibroblast cell line; Left panel) and Daudi (human Burkitt's lymphoma cell line; Right panel) cell lines labeling CD75 with ab225793 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control: HFF-1 [PMID: 23358684].
Immunoprecipitation - Anti-CD75 antibody [EPR22054-238] (ab225793)

CD75 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab225793 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225793 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Raji whole cell lysate 10 µg (Input).

Lane 2: ab225793 IP in Raji whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225793 in Raji whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds.
Anti-CD75 antibody [EPR22054-238] (ab225793)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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