Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD75 antibody [B-L5] - BSA and Azide free (ab269707)

Key features and details

  • Mouse monoclonal [B-L5] to CD75 - BSA and Azide free
  • Suitable for: Flow Cyt, IP
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-CD75 antibody [B-L5] - BSA and Azide free
    See all CD75 primary antibodies

  • Description

    Mouse monoclonal [B-L5] to CD75 - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    This antibody recognises CD75.

  • Tested Applications & Species

    Application Species
    IP
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Human CD75.

  • General notes

    ab269707 is a carrier free version of ab27316. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.3
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purification notes

    This antibody was purified by gel filtration.

  • Clonality

    Monoclonal

  • Clone number

    B-L5

  • Myeloma

    P3x63-Ag8.653

  • Isotype

    IgM

  • Research areas

Images

  • Flow Cytometry - Anti-CD75 antibody [B-L5] - BSA and Azide free (ab269707)
    Flow Cytometry - Anti-CD75 antibody [B-L5] - BSA and Azide free (ab269707)

    ab27316, at a dilution of 10µl for 106 cells or 100µl of whole blood, staining CD75 by Flow Cytometry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab27316).

  • Immunoprecipitation - Anti-CD75 antibody (ab269707)
    Immunoprecipitation - Anti-CD75 antibody (ab269707)

    CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to CD75 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 47kDa; CD75

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab27316).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD75 antibody [B-L5] - BSA and Azide free (ab269707)

© 2021 Astana Biomed Group LLP. All rights reserved.