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Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [OX49] to CD44 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Rat

Overview

  • Product name

    Anti-CD44 antibody [OX49] - BSA and Azide free
    See all CD44 primary antibodies

  • Description

    Mouse monoclonal [OX49] to CD44 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat

  • Immunogen

    Tissue, cells or virus corresponding to Rat CD44.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Rat spleen tissue. ICC/IF: C6 cells. Rat splenocytes.Flow Cyt: C6 cells.

  • General notes

    ab244580 is the PBS only version of ab238464.

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab238464).

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD44 with ab238464 at 0.085µg/ml, followed by a ready to use secondary. Membranous staining on rat spleen is observed. The section was incubated with ab238464 for 5 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)
    Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab238464).

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell) cells labeling CD44 with ab238464 at 0.854µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on C6 cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution.
    The negative controls are as follows:
    -ve control 1: ab238464 at 1/500 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)
    Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab238464).

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat splenocytes labeling CD44 with ab238464 at 0.854µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on rat splenocytes. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution.
    The negative controls are as follows:
    -ve control 1: ab238464 at 1/500 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Flow Cytometry - Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)
    Flow Cytometry - Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, Glycerol and sodium azide (ab238464).

    Flow cytometric analysis of C6 (Rat glial tumor glial cell) cell line labeling CD44 with ab238464 at 0.474µg/ml (red) compared with a Mouse monoclonal IgG Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

     

  • Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)
    Anti-CD44 antibody [OX49] - BSA and Azide free (ab244580)

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