Anti-CD75 antibody [B-L5] (ab27316)
![Anti-CD75 antibody [B-L5] (ab27316)](https://www.abcam.com/ps/products/27/ab27316/Images/ab27316-203457-IPVI001ab2731620m.jpg "Anti-CD75 antibody [B-L5] (ab27316)")
Key features and details
- Mouse monoclonal [B-L5] to CD75
- Suitable for: Flow Cyt, IP
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-CD75 antibody [B-L5]
See all CD75 primary antibodies -
Description
Mouse monoclonal [B-L5] to CD75 -
Host species
Mouse -
Specificity
This antibody recognises CD75. -
Tested applications
Suitable for: Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Raji cell line.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purification notes
This antibody was purified by gel filtration. -
Clonality
Monoclonal -
Clone number
B-L5 -
Myeloma
x63-Ag8.653 -
Isotype
IgM -
Research areas
Images
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Immunoprecipitation - Anti-CD75 antibody [B-L5] (ab27316)
CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to CD75 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 47kDa; CD75
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![Flow Cytometry - Anti-CD75 antibody [B-L5] (ab27316)](https://www.abcam.com/ps/products/27/ab27316/Images/ab27316.gif)
Flow Cytometry - Anti-CD75 antibody [B-L5] (ab27316)Ab27316, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD75 by Flow Cytometry.
