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Anti-CD75 antibody [B-L5] (ab27316)

Key features and details

  • Mouse monoclonal [B-L5] to CD75
  • Suitable for: Flow Cyt, IP
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-CD75 antibody [B-L5]
    See all CD75 primary antibodies

  • Description

    Mouse monoclonal [B-L5] to CD75

  • Host species

    Mouse

  • Specificity

    This antibody recognises CD75.

  • Tested applications

    Suitable for: Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Raji cell line.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.3
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purification notes

    This antibody was purified by gel filtration.

  • Clonality

    Monoclonal

  • Clone number

    B-L5

  • Myeloma

    x63-Ag8.653

  • Isotype

    IgM

  • Research areas

Images

  • Immunoprecipitation - Anti-CD75 antibody [B-L5] (ab27316)
    Immunoprecipitation - Anti-CD75 antibody [B-L5] (ab27316)

    CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to CD75 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 47kDa; CD75

  • Flow Cytometry - Anti-CD75 antibody [B-L5] (ab27316)
    Flow Cytometry - Anti-CD75 antibody [B-L5] (ab27316)
    Ab27316, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD75 by Flow Cytometry.

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