Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [GOT214A] to CD20 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD20 antibody [GOT214A] - BSA and Azide free
    See all CD20 primary antibodies

  • Description

    Rat monoclonal [GOT214A] to CD20 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse spleen tissue. ICC: Mouse PBMC cells.

  • General notes

    ab271298 is the carrier-free version of ab271288. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab271298 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)
    Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC cells labelling CD20 with ab271288 at 1/50 (16.04 μg/mL) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in some subset of mouse PBMC cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution(Red). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: Used ab271288 at 1/50 (16.04 μg/mL) dilution, followed by ab150080 at 1/500 dilution.

    -ve control 2: Used ab179513 at 1/200 dilution, followed by ab150157 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271288).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD20 with ab271288 at 1/100 (8.02 μg/mL) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Membranous staining on the mouse spleen is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271288).

  • Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)
    Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.