Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD21+CD35 antibody [4B2] (ab243317)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [4B2] to CD21+CD35
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CD21+CD35 antibody [4B2]
    See all CD21+CD35 primary antibodies

  • Description

    Mouse monoclonal [4B2] to CD21+CD35

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: 2.4G2 whole cell lysate. ICC/IF: WEHI-231 and 2.4G2 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)
    Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 2.4G2 (Rat B cell lymphoma B lymphocyte) cells labeling CD21 with ab243317 at 2.435µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in 2.4G2 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)
    Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (Mouse B cell lymphoma B lymphocyte) or EL4 (Mouse lymphoma T lymphocyte) cells labeling CD21 with ab243317 at 2.435µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and weak cytoplasmic staining in WEHI-231 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

    Negative control: EL4 (PMID: 10453011).

  • Western blot - Anti-CD21 antibody [4B2] (ab243317)
    Western blot - Anti-CD21 antibody [4B2] (ab243317)
    All lanes : Anti-CD21+CD35 antibody [4B2] (ab243317) at 0.487 µg/ml

    Lane 1 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
    Lane 2 : 2.4G2 (rat B cell lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

    Predicted band size: 113 kDa
    Observed band size: 155 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Negative control: EL4 (PMID: 10453011).

  • Anti-CD21+CD35 antibody [4B2] (ab243317)
    Anti-CD21+CD35 antibody [4B2] (ab243317)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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