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Anti-CD21+CD35 antibody [4B2] - BSA and Azide free (ab252778)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [4B2] to CD21+CD35 - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CD21+CD35 antibody [4B2] - BSA and Azide free
    See all CD21+CD35 primary antibodies

  • Description

    Mouse monoclonal [4B2] to CD21+CD35 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: 2.4G2 whole cell lysate. ICC/IF: WEHI-231 and 2.4G2 cells.

  • General notes

    ab252778 is a PBS only version of ab243317.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] - BSA and Azide free (ab252778)
    Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] - BSA and Azide free (ab252778)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 2.4G2 (Rat B cell lymphoma B lymphocyte) cells labeling CD21 with ab243317 at 2.435µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in 2.4G2 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab243317).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] - BSA and Azide free (ab252778)
    Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] - BSA and Azide free (ab252778)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (Mouse B cell lymphoma B lymphocyte) or EL4 (Mouse lymphoma T lymphocyte) cells labeling CD21 with ab243317 at 2.435µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and weak cytoplasmic staining in WEHI-231 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

    Negative control: EL4 (PMID: 10453011).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab243317).

  • Western blot - Anti-CD21 antibody [4B2] - BSA and Azide free (ab252778)
    Western blot - Anti-CD21 antibody [4B2] - BSA and Azide free (ab252778)
    All lanes : Anti-CD21+CD35 antibody [4B2] (ab243317) at 0.487 µg/ml

    Lane 1 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
    Lane 2 : 2.4G2 (rat B cell lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

    Predicted band size: 113 kDa
    Observed band size: 155 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Negative control: EL4 (PMID: 10453011).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and azide (ab243317).

  • Anti-CD21+CD35 antibody [4B2] - BSA and Azide free (ab252778)
    Anti-CD21+CD35 antibody [4B2] - BSA and Azide free (ab252778)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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