Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PGD antibody [EPR6565] (ab129199)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6565] to PGD
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PGD antibody [EPR6565]
    See all PGD primary antibodies

  • Description

    Rabbit monoclonal [EPR6565] to PGD

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PGD aa 400-500. The exact sequence is proprietary.

  • Positive control

    • WB: Raji, Molt-4, and 293T cell lysates. ICC/IF: HeLa cells. Flow Cyt: HEK293 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PGD antibody [EPR6565] (ab129199)
    Western blot - Anti-PGD antibody [EPR6565] (ab129199)
    All lanes : Anti-PGD antibody [EPR6565] (ab129199) at 1/5000 dilution

    Lane 1 : Mouse brain tissue lysates
    Lane 2 : Mouse heart tissue lysates
    Lane 3 : Mouse kidney tissue lysates
    Lane 4 : Mouse spleen tissue lysates
    Lane 5 : Rat brain tissue lysates
    Lane 6 : Rat heart tissue lysates
    Lane 7 : Rat kidney tissue lysates
    Lane 8 : Rat spleen tissue lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PGD antibody [EPR6565] (ab129199)
    Immunocytochemistry/ Immunofluorescence - Anti-PGD antibody [EPR6565] (ab129199)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PGD with ab129199 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Flow Cytometry - Anti-PGD antibody [EPR6565] (ab129199)
    Flow Cytometry - Anti-PGD antibody [EPR6565] (ab129199)

    Overlay histogram showing HEK293 cells stained with ab129199 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129199, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-PGD antibody [EPR6565] (ab129199)
    Western blot - Anti-PGD antibody [EPR6565] (ab129199)
    All lanes : Anti-PGD antibody [EPR6565] (ab129199) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Molt-4 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 53 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-PGD antibody [EPR6565] (ab129199)
    OI-RD Scanning - Anti-PGD antibody [EPR6565] (ab129199)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PGD antibody [EPR6565] (ab129199)
    Anti-PGD antibody [EPR6565] (ab129199)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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