Anti-Transketolase antibody (ab56199)
Key features and details
- Rabbit polyclonal to Transketolase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Transketolase antibody
See all Transketolase primary antibodies -
Description
Rabbit polyclonal to Transketolase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to C terminal residues of mouse Transketolase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by antigen specific affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Transketolase antibody (ab56199)Anti-Transketolase antibody (ab56199) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
The 74 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Transketolase.
