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Anti-Transketolase antibody [7H1AA1] (ab112997)

Key features and details

  • Mouse monoclonal [7H1AA1] to Transketolase
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Transketolase antibody [7H1AA1]
    See all Transketolase primary antibodies

  • Description

    Mouse monoclonal [7H1AA1] to Transketolase

  • Host species

    Mouse

  • Specificity

    Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • HeLa and HepG2 whole cell lysate (ab7900); HL60 and HeLa cells. Human normal colon FFPE tissue.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Transketolase antibody [7H1AA1] (ab112997)
    Western blot - Anti-Transketolase antibody [7H1AA1] (ab112997)
    All lanes : Anti-Transketolase antibody [7H1AA1] (ab112997) at 1 µg/ml

    Lane 1 : HepG2 Cell Lysate
    Lane 2 : HeLa Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 68 kDa

  • Flow Cytometry - Anti-Transketolase antibody [7H1AA1] (ab112997)
    Flow Cytometry - Anti-Transketolase antibody [7H1AA1] (ab112997)
    ab112997 at 1ug/ml staining Transketolase in HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-Transketolase antibody [7H1AA1] (ab112997)
    Immunocytochemistry/ Immunofluorescence - Anti-Transketolase antibody [7H1AA1] (ab112997)
    ab112997 at 1ug/ml staining Transketolase in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI (blue). Non specific fluorescence was reduced using a signal enhancer.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody [7H1AA1] (ab112997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transketolase antibody [7H1AA1] (ab112997)

    IHC image of Transketolase staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112997, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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