Anti-Collagen I antibody [COL-1] (ab6308)
![Anti-Collagen I antibody [COL-1] (ab6308)](https://www.abcam.com/ps/products/6/ab6308/Images/ab6308-12223-anti-collagen-i-antibody-col-1-immunohistochemistry.jpg "Anti-Collagen I antibody [COL-1] (ab6308)")
Key features and details
- Mouse monoclonal [COL-1] to Collagen I
- Suitable for: IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Collagen I antibody [COL-1]
See all Collagen I primary antibodies -
Description
Mouse monoclonal [COL-1] to Collagen I -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr HumanWB Human
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Immunogen
Full length native protein (purified) corresponding to Cow Collagen I. Cow skin collagen type I
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Epitope
The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding. There have been varying results when using this antibody in IHC-P. Please refer to our customer Abreviews for more protocol information and optimization steps when using this antibody in IHC-P. -
Positive control
- WB: Natural Cow Collagen I protein (ab7526), IHC-Fr: Human tonsil tissue.
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General notes
Production of this antibody has been changed on 23rd June 2016. The following lots are from ascites and are still in stock as of 23rd June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.0976% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from Tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
COL-1 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308) This image was kindly supplied as part of the review submitted by Arvi-Matti Kuusniemi
Immunoperoxidase staining of unfixed frozen tissue sections with ab6308. Picture of human kidney cortex showing two glomeruli and surrounding tubulointerstitium.
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![Western blot - Anti-Collagen I antibody [COL-1] (ab6308)](https://www.abcam.com/ps/products/6/ab6308/Images/ab6308-337140-anti-collagen-i-antibody-col-1-western-blot.jpg)
Western blot - Anti-Collagen I antibody [COL-1] (ab6308)Lane 1 : Anti-Collagen I antibody [COL-1] (ab6308) at 2 µg/ml
Lane 2 : Anti-Collagen I antibody [COL-1] (ab6308) at 1 µg/ml
Lane 3 : Anti-Collagen I antibody [COL-1] (ab6308) at 0.5 µg/ml
Lane 4 : Anti-Collagen I antibody [COL-1] (ab6308) at 0 µg/ml
All lanes : Recombinant human Collagen 1
Predicted band size: 130 kDa
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![Western blot - Anti-Collagen I antibody [COL-1] (ab6308)](https://www.abcam.com/ps/products/6/ab6308/Images/ab6308-12219-anti-collagen-i-antibody-col-1-western-blot.jpg)
Western blot - Anti-Collagen I antibody [COL-1] (ab6308) This image is courtesy of an anonymous AbreviewAnti-Collagen I antibody [COL-1] (ab6308) at 1/1000 dilution + Human brain whole tissue lysate at 10 µg
Secondary
An HRP-conjugated goat polyclonal to mouse IgG at 1/7500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 130 kDa
Exposure time: 24 hours
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![Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)](https://www.abcam.com/ps/products/6/ab6308/Images/ab6308-337141-anti-collagen-i-antibody-col-1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)Acetone-fixed human tonsil tissue frozen section stained for Collagen I with ab6308 at 7 μg/mL in immunohistochemical analysis.
