Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22894-89] to Collagen I - BSA and Azide free
  • Suitable for: ICC/IF, IP, Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free
    See all Collagen I primary antibodies

  • Description

    Rabbit monoclonal [EPR22894-89] to Collagen I - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt (Intra), WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HFF-1 and NIH/3T3 cells. WB: HFF-1 and NIH/3T3 whole cell lysate. Rat lung organ culture supernatant. Human lung tissue lysate. IP: Mouse lung lysate. Flow Cyt (intra): HFF-1 and NIH/3T3 cells.

  • General notes

    ab264074 is the carrier-free version of ab260043.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)
    Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HFF-1 (Human skin fibroblast) cells labeling Collagen I with ab260043 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HFF-1 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)
    Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling Collagen I with ab260043 at 1/250 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

  • Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)
    Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)

    Collagen I was immunoprecipitated from 0.35 mg mouse lung lysate 10µg with ab260043 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260043 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse lung lysate 10µg

    Lane 2: ab260043 IP in mouse lung lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260043 in mouse lung lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

  • Flow Cytometry - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)
    Flow Cytometry - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling Collagen I with ab260043 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

  • Flow Cytometry - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)
    Flow Cytometry - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HFF-1 (Human skin fibroblast) cells labeling Collagen I with ab260043 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

  • Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)
    Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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