HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)
![HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)](https://www.abcam.com/ps/products/197/ab197721/Images/ab197721-312097-anti-hp1-alpha-antibody-epr5777-heterochromatin-marker-hrp-western-blot.jpg "HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker
See all HP1 alpha primary antibodies -
Description
HRP Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, SH-SY5Y, MCF7, A431 cell lysates and Human tonsil tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5777 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)All lanes : HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CBX5 (HP1 alpha) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 22 kDa
Exposure time: 20 minutesab197721 was shown to specifically react with HP1 alpha in wild-type HAP1 cells as signal was lost in CBX5 (HP1 alpha) knockout cells. Wild-type and CBX5 (HP1 alpha) knockout samples were subjected to SDS-PAGE. Ab197721 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)](https://www.abcam.com/ps/products/197/ab197721/Images/ab197721-283849-anti-hp1-alpha-antibody-epr5777-heterochromatin-marker-hrp-western-blot.jpg)
Western blot - HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)All lanes : HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721) at 1/5000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 5 : Hap1 WT at 20 µg
Lane 6 : HP1 alpha knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197721 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)](https://www.abcam.com/ps/products/197/ab197721/Images/ab197721-3-benefits-of-recombinant-antibodies.png)
HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)
