Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Anti-HP1 alpha antibody [EPR5776] (ab133577)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5776] to HP1 alpha
Suitable for: Flow Cyt, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-HP1 alpha antibody [EPR5776]
See all HP1 alpha primary antibodies
Description

Rabbit monoclonal [EPR5776] to HP1 alpha
Host species

Rabbit
Specificity

The immunogen used for this product shares 83% homology with CBX1/HP1 beta. Cross-reactivity with this protein has not been confirmed experimentally.
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to a region within Human HP1 alpha (UniProt P45973).
Positive control
- SH-SY5Y cells; HeLa, SH-SY5Y, MCF7, A431, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5776
Isotype

IgG
Research areas

Western blot - Anti-HP1 alpha antibody [EPR5776] (ab133577)

All lanes : Anti-HP1 alpha antibody [EPR5776] (ab133577) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 22 kDa
Flow Cytometry - Anti-HP1 alpha antibody [EPR5776] (ab133577)

Flow cytometrric analysis of HPA1 alpha in permeabilized SH SY5Y cells, using ab133577 at a 1/10 dilution (red) or a control rabbit IgG antibody (green).
Anti-HP1 alpha antibody [EPR5776] (ab133577)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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