Anti-HP1 alpha antibody [3A11F8] (ab234085)
Key features and details
- Mouse monoclonal [3A11F8] to HP1 alpha
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-HP1 alpha antibody [3A11F8]
See all HP1 alpha primary antibodies -
Description
Mouse monoclonal [3A11F8] to HP1 alpha -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB HumanRecombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human HP1 alpha aa 1 to the C-terminus. Expressed in E. coli.
Database link: P45973 -
Positive control
- WB: HeLa, NIH/3T3, K562, MCF7, Jurkat and A431 cell lysate; Recombinant human HP1 alpha (amino acids: 1-191) protein; HP1 alpha (amino acids: 1-191)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa cells. Flow Cytometry: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3A11F8 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 6 : A431 (human epidermoid carcinoma cell line) cell lysate
Predicted band size: 22 kDa
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HP1 alpha (green) using ab234085 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AlexaFluor®555-Phalloidin.
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Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling HP1 alpha with ab234085 at 1/200 dilution (green) compared to a negative control (red).
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All lanes : Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : HP1 alpha (amino acids: 1-191)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 22 kDa
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Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution + Recombinant human HP1 alpha (amino acids: 1-191) protein
Predicted band size: 22 kDaExpected recombinant MW is 48.2 kDa