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Epigenetics and Nuclear Signaling Chromatin Binding Proteins Methyl Lysine

Anti-HP1 alpha antibody [3A11F8] (ab234085)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-HP1 alpha antibody [3A11F8] (ab234085)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [3A11F8] to HP1 alpha
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-HP1 alpha antibody [3A11F8]
    See all HP1 alpha primary antibodies
  • Description

    Mouse monoclonal [3A11F8] to HP1 alpha
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human HP1 alpha aa 1 to the C-terminus. Expressed in E. coli.
    Database link: P45973

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HeLa, NIH/3T3, K562, MCF7, Jurkat and A431 cell lysate; Recombinant human HP1 alpha (amino acids: 1-191) protein; HP1 alpha (amino acids: 1-191)-hIgGFc transfected HEK-293 cell lysate. ICC/IF: HeLa cells. Flow Cytometry: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    3A11F8
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Methyl Lysine
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Chromatin

Images

  • Western blot - Anti-HP1 alpha antibody [3A11F8] (ab234085)
    Western blot - Anti-HP1 alpha antibody [3A11F8] (ab234085)
    All lanes : Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 6 : A431 (human epidermoid carcinoma cell line) cell lysate

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [3A11F8] (ab234085)
    Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [3A11F8] (ab234085)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HP1 alpha (green) using ab234085 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AlexaFluor®555-Phalloidin. 

  • Flow Cytometry - Anti-HP1 alpha antibody [3A11F8] (ab234085)
    Flow Cytometry - Anti-HP1 alpha antibody [3A11F8] (ab234085)

    Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling HP1 alpha with ab234085 at 1/200 dilution (green) compared to a negative control (red).

  • Western blot - Anti-HP1 alpha antibody [3A11F8] (ab234085)
    Western blot - Anti-HP1 alpha antibody [3A11F8] (ab234085)
    All lanes : Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : HP1 alpha (amino acids: 1-191)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 22 kDa

  • Western blot - Anti-HP1 alpha antibody [3A11F8] (ab234085)
    Western blot - Anti-HP1 alpha antibody [3A11F8] (ab234085)
    Anti-HP1 alpha antibody [3A11F8] (ab234085) at 1/100 dilution + Recombinant human HP1 alpha (amino acids: 1-191) protein

    Predicted band size: 22 kDa



    Expected recombinant MW is 48.2 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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