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Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab202533)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 568 Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker
  • Suitable for: ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm

Overview

  • Product name

    Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker
    See all HP1 alpha primary antibodies

  • Description

    Alexa Fluor® 568 Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MCF7 cells, HAP1-CBX5 WT and KO

  • General notes

    Alternative versions available:
    Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028) - Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab202533)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab202533)

    ab202533 staining HP1α in wild-type HAP1 cells (top panel) and HP1α in wild-type HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab202533 at 1/500 dilution (shown in pseudo colour green) and ab7291 at 1μg/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 647) (ab150119) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

    This product also gave a positive signal under the same testing conditions in HAP1 cells fixed with 4% formaldehyde (10 min).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab202533)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab202533)

    ab202533 staining HP1 alpha in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202533 at 1/200 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5min).

  • Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab202533)
    Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab202533)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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