Anti-HP1 alpha antibody (ab77256)
Key features and details
- Goat polyclonal to HP1 alpha
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HP1 alpha antibody
See all HP1 alpha primary antibodies -
Description
Goat polyclonal to HP1 alpha -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Rhesus monkey, Chinese hamster
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Immunogen
Synthetic peptide:
C-NKRKSNFSNSADDIK
, corresponding to internal sequence amino acids 88-102 of Human HP1 alpha (NP_036249.1). -
Positive control
- WB: K562, MCF7 and NIH3T3 cell lysates. IHC-P: Human uterus tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab77256 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HP1 alpha antibody (ab77256)All lanes : Anti-HP1 alpha antibody (ab77256) at 0.3 µg/ml
Lane 1 : A431 cell line lysates in RIPA buffer
Lane 2 : HeLa cell line lysates in RIPA buffer
Lane 3 : NIH3T3 cell line lysates in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 22 kDaPrimary incubation at room temperature for 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 alpha antibody (ab77256)ab77256 (2µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
