Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 1 ng/well
Overview
-
Product name
Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric)
See all Histone H4 kits -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Sensitivity
1 ng/well -
Range
10 ng/well - 2000 ng/well -
Assay time
2h 30m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Mammals
-
Product overview
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of proper higher order structure of DNA and is consequently essential for chromosome condensation, as well as functioning in gene silencing. H4 (di-methyl K20) has been described as another repressive chromatin domain and is involved in the DNA damage response. H4 (tri-methyl K20) acts as a passive feature or structure determinant for chromatin degradation and release, as well as being an epigenetic marker of early apoptosis. H4 (tri-methyl K20) is also considered as a common hallmark of human cancer.
Abcam's Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100) enables the user to measure global mono-, di- and tri-methylation of histone H4K20. The global H4K20 pan-methylation can also be changed by inhibiton or activation of HMTs, making quantitative detection of global pan-methyl histone H4K20 a useful tool for better understanding epigenetic regulation of gene activation/repression. ab115100 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
-
Platform
Microplate reader
Properties
-
Storage instructions
Please refer to protocols. -
Components Identifier 96 tests 10X Wash Buffer 1 x 20ml 8 Well Sample Strip (with Frame) 1 x 9 units 8 Well Standard Control Strips Green Ringed 1 x 3 units Antibody Buffer 1 x 12ml Detecting Antibody, 1 mg/mL 1 x 10µl Fluoro Developer 1 x 24µl Fluoro Dilution 1 x 8ml Fluoro Enhancer 1 x 24µl Signal Enhancer 1 x 240µl Signal Report Solution 1 x 10µl Standard Control, 100 µg/mL 1 x 20µl -
Research areas
-
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
-
Alternative names
- dJ160A22.1
- dJ160A22.2
- dJ221C16.1
see all -
Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8368 Human
- Entrez Gene: 8370 Human
see all