Human Complement C5a ELISA Kit (ab193695)
Key features and details
- Sensitivity: 31 pg/ml
- Range: 31 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Complement C5a ELISA Kit
See all C5 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
31 pg/ml -
Range
31 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 127.1 122% - 138% Plasma 122.5 114% - 130% Cell culture media 92.43 70% - 104% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Complement C5a Human ELISA Kit (ab193695) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human C5A in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human C5a coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures C5a present in the samples. The wells are washed and biotinylated anti-Human C5a antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of C5a bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated Human C5a detection antibody (lyophilized) 2 vials Human C5a Standards (lyophilized) 2 vials Pre-coated Human C5a Microplate (12 Strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). -
Involvement in disease
Defects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705). -
Sequence similarities
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Anaphylatoxin C5a analog
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
- C5
see all -
Database links
- Entrez Gene: 727 Human
- Omim: 120900 Human
- SwissProt: P01031 Human
- Unigene: 494997 Human
Images
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Standard Curve.
Standard Curve in Assay Diluent A using ab193695 Complement C5a Human ELISA Kit.
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Standard Curve.Standard Curve in Assay Diluent B using ab193695 Complement C5a Human ELISA Kit.
