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Immunology Innate Immunity Macrophage / Inflamm.

Anti-TREM2 antibody [EPR23177-106] (ab245227)

Price and availability

321 638 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-TREM2 antibody [EPR23177-106] (ab245227)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23177-106] to TREM2
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-TREM2 antibody [EPR23177-106]
    See all TREM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR23177-106] to TREM2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 267.4 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23177-106
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.

Images

  • Flow Cytometry - Anti-TREM2 antibody [EPR23177-106] (ab245227)
    Flow Cytometry - Anti-TREM2 antibody [EPR23177-106] (ab245227)

    Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling Trem2 with ab245227 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Neuro-2a (PMID: 19302484).

    Gated on viable cells.

    Brighter secondary antibody like Alexa Fluor® 647 is required to get desirable staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR23177-106] (ab245227)
    Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR23177-106] (ab245227)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Trem2 with ab245227 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and staining in RAW 264.7 cell line. Negative control: Neuro-2a (PMID: 19302484). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Anti-TREM2 antibody [EPR23177-106] (ab245227)
    Anti-TREM2 antibody [EPR23177-106] (ab245227)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-TREM2 antibody [EPR23177-106] (ab245227)

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    Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)

    Applications: Flow Cyt, ICC/IF

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    Applications: IP, WB

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    Anti-TREM2 antibody [EPR20243] - BSA and Azide free (ab232470)

    Applications: IP, WB

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    Anti-TREM2 antibody [6E9] (ab125117)

    Applications: Flow Cyt

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    Anti-TREM2 antibody [RM0139-5J46] (ab86491)

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    FITC Anti-TREM2 antibody [6E9] (ab236286)

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