Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23177-106] to TREM2 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse

Overview

  • Product name

    Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free
    See all TREM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23177-106] to TREM2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells.

  • General notes

    ab269868 is the carrier-free version of ab245227. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23177-106

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)
    Flow Cytometry - Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)

    Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling Trem2 with ab245227 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Neuro-2a (PMID: 19302484).

    Gated on viable cells.

    Brighter secondary antibody like Alexa Fluor® 647 is required to get desirable staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245227).

  • Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)
    Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Trem2 with ab245227 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and staining in RAW 264.7 cell line. Negative control: Neuro-2a (PMID: 19302484). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245227).

  • Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)
    Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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