Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)
![Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)](https://www.abcam.com/ps/products/269/ab269868/Images/ab269868-2-anti-trem2-antibody-epr23177-106-flowcytometry-raw-2647-neuro2a-mouse.jpg "Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23177-106] to TREM2 - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
Overview
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Product name
Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free
See all TREM2 primary antibodies -
Description
Rabbit monoclonal [EPR23177-106] to TREM2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells.
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General notes
ab269868 is the carrier-free version of ab245227. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23177-106 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)
Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling Trem2 with ab245227 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
Negative control: Neuro-2a (PMID: 19302484).
Gated on viable cells.
Brighter secondary antibody like Alexa Fluor® 647 is required to get desirable staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245227).
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![Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)](https://www.abcam.com/ps/products/269/ab269868/Images/ab269868-1-anti-trem2-antibody-epr23177-106-immunocytochemistry-raw-2647-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Trem2 with ab245227 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and staining in RAW 264.7 cell line. Negative control: Neuro-2a (PMID: 19302484). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245227).
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![Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)](https://www.abcam.com/ps/products/269/ab269868/Images/ab269868-3-benefits-of-recombinant-antibodies.png)
Anti-TREM2 antibody [EPR23177-106] - BSA and Azide free (ab269868)
