Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TREM2 antibody [RM0139-5J46] (ab86491)

Key features and details

  • Rat monoclonal [RM0139-5J46] to TREM2
  • Suitable for: ICC
  • Reacts with: Mouse
  • Isotype: IgG2b

Overview

  • Product name

    Anti-TREM2 antibody [RM0139-5J46]
    See all TREM2 primary antibodies

  • Description

    Rat monoclonal [RM0139-5J46] to TREM2

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC
    Mouse
    See all applications and species data

  • Immunogen

    Mouse recombinant protein of the extracellular EC-domain of TREM2

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    The IgG fraction of culture supernatant was purified by Protein A affinity chromatography.

  • Clonality

    Monoclonal

  • Clone number

    RM0139-5J46

  • Isotype

    IgG2b

  • Research areas

Images

  • Immunocytochemistry - Anti-TREM2 antibody [RM0139-5J46] (ab86491)
    Immunocytochemistry - Anti-TREM2 antibody [RM0139-5J46] (ab86491)
    Immunocytochemistry analysis of mouse brain microglia cells labeling TREM2 antibody with ab86491.

     

  • Immunohistochemistry (Frozen sections) - Anti-TREM2 antibody [RM0139-5J46] (ab86491)
    Immunohistochemistry (Frozen sections) - Anti-TREM2 antibody [RM0139-5J46] (ab86491) This image is courtesy of an abreview submitted by Dr Ruma Raha-Chowdhury.

    IHC-Fr image of human blood vessels stained with Ab86491 (1:250). The fixed brain sections were permeabilized using 0.1% PBS-Triton X for 1h. 10% donkey serum was used for 1 hour at 24°C to block protein-protein interactions.  The sections were then incubated with Ab8649 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-rat used at 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

  • Immunocytochemistry - Anti-TREM2 antibody [RM0139-5J46] (ab86491)
    Immunocytochemistry - Anti-TREM2 antibody [RM0139-5J46] (ab86491) This image is courtesy of an abreview submitted by Dr Ruma Raha-Chowdhury.

    Immunofluorescence analysis of 18 day rat embroyonic hypothalmic neurones labelling TREM2 with ab86491. Embryonic hypothalmic neuronal cells were cultured in a neurobasal media with B27 culture suppliment for three weeks and fixed with 2%PFA. A rat monoclonal Alexa fluoro® 568 (red) secondary was used at a concentration of 1/1000. Synaptophysin was counterstained with an Alexa fluoro® 488 (green) and nuclear DNA was stained with DAPI (blue).

     

    See Abreview

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