Anti-TREM2 antibody - C-terminal (ab223684)
Key features and details
- Goat polyclonal to TREM2 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TREM2 antibody - C-terminal
See all TREM2 primary antibodies -
Description
Goat polyclonal to TREM2 - C-terminal -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human TREM2 aa 150 to the C-terminus (C terminal) (Cysteine residue). NP_061838.1.
Database link: Q9NZC2 -
Positive control
- WB: Human cerebellum, frontal cortex and hippocampus lysates. IHC-P: Human spleen tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223684 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TREM2 antibody - C-terminal (ab223684)All lanes : Anti-TREM2 antibody - C-terminal (ab223684) at 0.3 µg/ml
Lane 1 : Human cerebellum lysate in RIPA buffer
Lane 2 : Human frontal cortex lysate in RIPA buffer
Lane 3 : Human hippocampus lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody - C-terminal (ab223684)Paraffin-embedded human spleen tissue stained for TREM2 using ab223684 at 5 µg/ml in immunohistochemical analysis, followed by AP-staining.
This data was obtained using a previous batch.
