Antibodies, Proteins, Kits and Reagents for Life Science

308 236 ₸ Product size

100 µl 308 236 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TREM2 antibody [EPR20243] (ab209814)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20243] to TREM2
Suitable for: IP, WB
Reacts with: Human

Product name

Anti-TREM2 antibody [EPR20243]
See all TREM2 primary antibodies
Description

Rabbit monoclonal [EPR20243] to TREM2
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: THP-1 whole cell lysate treated with 100ng/ml Phorbol-12-myristate-13-acetate (PMA) for 72 hours. IP: THP-1 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20243
Isotype

IgG
Research areas

Western blot - Anti-TREM2 antibody [EPR20243] (ab209814)

All lanes : Anti-TREM2 antibody [EPR20243] (ab209814) at 1/1000 dilution

Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1 whole cell lysate treated with 100ng/ml Phorbol-12-myristate-13-acetate (PMA) for 72 hours

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 25 kDa
Observed band size: 26 kDa
why is the actual band size different from the predicted?

Exposure time: 4 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-TREM2 antibody [EPR20243] (ab209814)

TREM2 was immunoprecipitated from 0.35 mg of THP-1 (Human monocytic leukemia cell line) whole cell lysate with ab209814 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209814 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: THP-1 whole cell lysate, 10 μg (Input).

Lane 2: ab209814 IP in THP-1 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209814 in THP-1 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-TREM2 antibody [EPR20243] (ab209814)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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