Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9522] to Mast Cell Tryptase - Low endotoxin, Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free
    See all Mast Cell Tryptase primary antibodies

  • Description

    Rabbit monoclonal [EPR9522] to Mast Cell Tryptase - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to Human Mast Cell Tryptase.

  • Positive control

    • Human skin, colon cancer or tonsil lysate, Human colon tissue. IP:Human skin lysate.

  • General notes

    ab216451 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)

    Immunohistochemical analysis of paraffin embedded Human tissue labeling Mast Cell Tryptase with ab151757 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151757).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)
    Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)

    This data was developed using ab151757, the same antibody clone in a different buffer formulation.
    Purified ab151757 at 1/50 dilution (2µg) immunoprecipitating Mast Cell Tryptase in Human skin lysate.
    Lane 1 (input): Human skin lysate 10µg
    Lane 2 (+): ab151757 + Human skin lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151757 in Human skin lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 30 kDa

  • Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)
    Anti-Mast Cell Tryptase antibody [EPR9522] - Low endotoxin, Azide free (ab216451)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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