Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8475] to Mast Cell Tryptase - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free
    See all Mast Cell Tryptase primary antibodies

  • Description

    Rabbit monoclonal [EPR8475] to Mast Cell Tryptase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human colon and tonsil tissues.

  • General notes

    ab227573 is the carrier-free version of ab134931 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab227573 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling Mast Cell Tryptase using ab134931 at a 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134931).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)

    Immunohistochemical analysis using ab134931 showing positive staining in Normal stomach tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134931).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)

    Immunohistochemical analysis using ab134931 showing positive staining in Colonic adenocarcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134931).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)

    This IHC data was generated using the same anti-Mast Cell Tryptase antibody clone, EPR8475, in a different buffer formulation (cat# ab134931).

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human tonsil tissue, labelling Mast Cell Tryptase using ab134931 at a 1/500 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)

    This IHC data was generated using the same anti-Mast Cell Tryptase antibody clone, EPR8475, in a different buffer formulation (cat# ab134931).

    Immunohistochemical analysis using ab134931 showing positive staining in Breast carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)
    Anti-Mast Cell Tryptase antibody [EPR8475] - BSA and Azide free (ab227573)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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