Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8476] to Mast Cell Tryptase - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free
    See all Mast Cell Tryptase primary antibodies

  • Description

    Rabbit monoclonal [EPR8476] to Mast Cell Tryptase - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Lab re-tests in mouse and rat species were negative. Therefore, we do not gaurantee this antibody in these species.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human stomach, colon and tonsil tissues.

  • General notes

    Ab227572 is the carrier-free version of ab134932. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab227572 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling Mast Cell Tryptase with Purified ab134932 at 1:5000 dilution (0.65 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134932)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572) This image is courtesy of an anonymous Abreview.

    ab14404 staining Mast Cell Tryptase in Mouse small intestine tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Cells were fixed with paraformaldehyde, blocked with 5% serum for 60 minutes at 21°C and antigen retrieval was by heat mediation in 10mM Citrate buffer pH6.0. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit Ipolyclonal(1/400) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134932).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)

    This IHC data was generated using the same anti-Mast Cell Tryptase antibody clone, EPR8476, in a different buffer formulation (cat# ab134932).

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human tonsil tissue, labelling Mast Cell Tryptase using ab134932 at a 1/500 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)

    This IHC data was generated using the same anti-Mast Cell Tryptase antibody clone, EPR8476, in a different buffer formulation (cat# ab134932).

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling Mast Cell Tryptase using ab134932 at a 1/500 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)
    Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Mast Cell Tryptase antibody [EPR8476] - BSA and Azide free (ab227572)

© 2021 Astana Biomed Group LLP. All rights reserved.