Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸ 10 µl 140 716 ₸

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Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9522] to Mast Cell Tryptase
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-Mast Cell Tryptase antibody [EPR9522]
See all Mast Cell Tryptase primary antibodies
Description

Rabbit monoclonal [EPR9522] to Mast Cell Tryptase
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Mast Cell Tryptase aa 1-300.
Positive control
- Human skin, colon cancer or tonsil lysate, Human colon tissue. IP: Human skin lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9522
Isotype

IgG
Research areas

Western blot - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)

All lanes : Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757) at 1/1000 dilution

Lane 1 : Human skin lysate
Lane 2 : Human colon cancer lysate
Lane 3 : Human tonsil lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 30 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)

Immunohistochemical analysis of paraffin embedded Human tissue labeling Mast Cell Tryptase with ab151757 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)

Purified ab151757 at 1/50 dilution (2µg) immunoprecipitating Mast Cell Tryptase in Human skin lysate.
Lane 1 (input): Human skin lysate 10µg
Lane 2 (+): ab151757 + Human skin lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151757 in Human skin lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 30 kDa
Anti-Mast Cell Tryptase antibody [EPR9522] (ab151757)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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