Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VCAM1 antibody [EPR17010-83] (ab181315)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17010-83] to VCAM1
  • Suitable for: IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-VCAM1 antibody [EPR17010-83]
    See all VCAM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17010-83] to VCAM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse VCAM1 active protein; LADMAC, NIH/3T3, and LPS treated bEnd.3 whole cell lysates; Mouse spleen and bone marrow lysates. IP: NIH/3T3 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-VCAM1 antibody [EPR17010-83] (ab181315)
    Western blot - Anti-VCAM1 antibody [EPR17010-83] (ab181315)
    All lanes : Anti-VCAM1 antibody [EPR17010-83] (ab181315) at 1/1000 dilution

    Lane 1 : LADMAC (Mouse bone marrow monocyte macrophage) whole cell lysate
    Lane 2 : bEnd.3 (Mouse brain endothelioma) whole cell lysate
    Lane 3 : bEnd.3 (Mouse brain endothelioma) treated with 10 µg/ml LPS for 24 h, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 50 seconds


    Rabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.

    Blocking/Diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-VCAM1 antibody [EPR17010-83] (ab181315)
    Western blot - Anti-VCAM1 antibody [EPR17010-83] (ab181315)
    Anti-VCAM1 antibody [EPR17010-83] (ab181315) at 1/5000 dilution + Mouse VCAM1 active protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse VCAM1 active protein contains aa1-698.

  • Western blot - Anti-VCAM1 antibody [EPR17010-83] (ab181315)
    Western blot - Anti-VCAM1 antibody [EPR17010-83] (ab181315)
    All lanes : Anti-VCAM1 antibody [EPR17010-83] (ab181315) at 1/1000 dilution

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Mouse bone marrow lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 81 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2: 15 seconds.

  • Immunoprecipitation - Anti-VCAM1 antibody [EPR17010-83] (ab181315)
    Immunoprecipitation - Anti-VCAM1 antibody [EPR17010-83] (ab181315)

    VCAM1 was immunoprecipitated from 1 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab181315 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab181315 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10µg (Input).

    Lane 2: ab181315 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab181315 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

  • Anti-VCAM1 antibody [EPR17010-83] (ab181315)
    Anti-VCAM1 antibody [EPR17010-83] (ab181315)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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