Anti-TREM1 antibody (ab93717)
Key features and details
- Rabbit polyclonal to TREM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TREM1 antibody
See all TREM1 primary antibodies -
Description
Rabbit polyclonal to TREM1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human TREM1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab107110) -
Positive control
- This antibody gave a positive signal in both Human liver and Human Fetal liver tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).Target
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Function
Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. -
Tissue specificity
Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 54210 Human
- Omim: 605085 Human
- SwissProt: Q9NP99 Human
- Unigene: 283022 Human
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Alternative names
- CD354 antibody
- OTTMUSP00000018206 antibody
- TREM 1 antibody
see all
Images
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Western blot - Anti-TREM1 antibody (ab93717)All lanes : Anti-TREM1 antibody (ab93717) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue (ab29890)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThe band observed at 22 kDa could potentially be a cleaved form of TREM1 due to the presence of a 20 amino acid signal peptide.
Protocols
Datasheets and documents
References (2)
ab93717 has been referenced in 2 publications.
- Feng JY et al. Role of TREM-1 in pulmonary tuberculosis patients- analysis of serum soluble TREM-1 levels. Sci Rep 8:8223 (2018). PubMed: 29844416
- Canavan M et al. Enriched Cd141+ DCs in the joint are transcriptionally distinct, activated, and contribute to joint pathogenesis. JCI Insight 3:N/A (2018). PubMed: 30518680
Images
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Western blot - Anti-TREM1 antibody (ab93717)All lanes : Anti-TREM1 antibody (ab93717) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue (ab29890)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThe band observed at 22 kDa could potentially be a cleaved form of TREM1 due to the presence of a 20 amino acid signal peptide.
