Anti-Olig1 antibody (ab139512)
Key features and details
- Rabbit polyclonal to Olig1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Olig1 antibody
See all Olig1 primary antibodies -
Description
Rabbit polyclonal to Olig1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Synthetic peptide within Mouse Olig1 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9JKN5 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cerebellum; Rat Cerebellum; Rat Oligodendrocyte Precursor Cells (OPC).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatAll applications PigApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 27 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 27 kDa).Target
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Function
Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube. -
Tissue specificity
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 50914 Mouse
- Entrez Gene: 60394 Rat
- SwissProt: Q9JKN5 Mouse
- SwissProt: Q9WUQ3 Rat
- Unigene: 39300 Mouse
- Unigene: 45339 Rat
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Alternative names
- Basic domain helix loop helix protein class B 6 antibody
- Basic domain helix loop helix protein class B6 antibody
- BHLH B6 antibody
see all
Images
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Western blot - Anti-Olig1 antibody (ab139512)All lanes : Anti-Olig1 antibody (ab139512) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Rat Oligodendrocyte Precursor Cells
Lane 4 : Cerebellum (Mouse) Tissue Lysate
Lane 5 : Cerebellum (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 14 kDa (possible non-specific binding), 20 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab139512 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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Western blot - Anti-Olig1 antibody (ab139512)All lanes : Anti-Olig1 antibody (ab139512) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Cerebellum (Mouse) Tissue Lysate
Lane 4 : Cerebellum (Rat) Tissue Lysate
Lane 5 : Brain (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 6 : Brain (Rat) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 7 : Cerebellum (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 8 : Cerebellum (Rat) Tissue Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab139512 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Datasheets and documents
References (0)
ab139512 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Olig1 antibody (ab139512)All lanes : Anti-Olig1 antibody (ab139512) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Rat Oligodendrocyte Precursor Cells
Lane 4 : Cerebellum (Mouse) Tissue Lysate
Lane 5 : Cerebellum (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 14 kDa (possible non-specific binding), 20 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab139512 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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Western blot - Anti-Olig1 antibody (ab139512)All lanes : Anti-Olig1 antibody (ab139512) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Cerebellum (Mouse) Tissue Lysate
Lane 4 : Cerebellum (Rat) Tissue Lysate
Lane 5 : Brain (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 6 : Brain (Rat) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 7 : Cerebellum (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 8 : Cerebellum (Rat) Tissue Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab139512 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
