Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspr antibody [K65/35] (ab252535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [K65/35] to Caspr
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caspr antibody [K65/35]
    See all Caspr primary antibodies

  • Description

    Mouse monoclonal [K65/35] to Caspr

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Rat
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment within Rat Caspr aa 1300 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P97846

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Mouse and rat brain lysate. IHC-P: Human, mouse and rat cerebrum tissue. ICC/IF: HeLa, C6 and Neuro-2a cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    K65/35

  • Isotype

    IgG2b

  • Research areas

Images

  • Western blot - Anti-Caspr antibody [K65/35] (ab252535)
    Western blot - Anti-Caspr antibody [K65/35] (ab252535)
    All lanes : Anti-Caspr antibody [K65/35] (ab252535) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 156 kDa
    Observed band size: 220 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 7.75 seconds.

    Caspr protein (220 kd) was observed. The molecular weight is consistent with that has been described in the literature (PMID: 20610764).

    We recommend to use 1% SDS Hot lysis method to get desired WB results.  For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] (ab252535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] (ab252535)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Caspr with ab252535 at 1/10000 dilution, followed by ready to use secondary antibody. Positive staining on rat cerebrum is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252535 for 10 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] (ab252535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] (ab252535)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Caspr with ab252535 at 1/10000 dilution, followed by ready to use secondary antibody. Positive staining on mouse cerebrum is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252535 for 10 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] (ab252535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] (ab252535)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Caspr with ab252535 at 1/10000 dilution, followed by ready to use secondary antibody. Positive staining on human cerebrum is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252535 for 10 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] (ab252535)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] (ab252535)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell line) cells labeling Caspr with ab252535 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in C6 cell line. The nuclear counter stain is DAPI (Blue). Tubulin is detected using ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    The negative controls are as follows:
    -ve control 1: ab252535 at 1/50 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] (ab252535)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] (ab252535)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling Caspr with ab252535 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. The nuclear counter stain is DAPI (Blue). Tubulin is detected using ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    The negative controls are as follows:
    -ve control 1: ab252535 at 1/50 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] (ab252535)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] (ab252535)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Caspr with ab252535 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counter stain is DAPI (Blue). Tubulin is detected using ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    The negative controls are as follows:
    -ve control 1: ab252535 at 1/50 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Anti-Caspr antibody [K65/35] (ab252535)
    Anti-Caspr antibody [K65/35] (ab252535)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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