Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2673] to Olig2 - BSA and Azide free
  • Suitable for: ICC, Mass Cytometry, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Olig2 antibody [EPR2673] - BSA and Azide free
    See all Olig2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2673] to Olig2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Rat
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Oligodendroglioma lysate, human glioma tissue; IMC: Human glioblastoma brain cancer tissue; ICC: Rat primary glia cells.

  • General notes

    Ab220796 is the carrier-free version of ab109186. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab220796 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    Immunocytochemistry - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab109186)

    ab109186 staining Olig2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab109186 at 1?g/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Mass Cytometry - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    Mass Cytometry - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796) This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University

    Imaging Mass Cytometry™ (IMC™) image of human glioblastoma brain cancer tissue stained with Anti-Olig2 antibody [EPR2673]. ab220796 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.

    Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

  • Immunocytochemistry - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    Immunocytochemistry - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

    This data was developed using ab109186, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary glia cell cells labelling Olig2 with ab109186 at 1/100 (1.23 µg/mL) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in rat primary glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

    Immunohistochemical staining of Olig2 in human glioma tissue with ab109186 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    OI-RD Scanning - Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109186).

  • Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)

© 2021 Astana Biomed Group LLP. All rights reserved.