Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [K65/35] to Caspr - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caspr antibody [K65/35] - BSA and Azide free
    See all Caspr primary antibodies

  • Description

    Mouse monoclonal [K65/35] to Caspr - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Rat Caspr aa 1300 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P97846

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Mouse and rat brain lysate. IHC-P: Human, mouse and rat cerebrum tissue. ICC/IF: HeLa, C6 and Neuro-2a cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    ab255761 is the carrier-free version of ab252535. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255761 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    K65/35

  • Isotype

    IgG2b

  • Research areas

Images

  • Western blot - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)
    Western blot - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)
    All lanes : Anti-Caspr antibody [K65/35] (ab252535) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 156 kDa
    Observed band size: 220 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 7.75 seconds.

    Caspr protein (220 kd) was observed. The molecular weight is consistent with that has been described in the literature (PMID: 20610764).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (252535).

    We recommend to use 1% SDS Hot lysis method to get desired WB results.  For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Caspr with ab252535 at 1/10000 dilution, followed by ready to use secondary antibody. Positive staining on rat cerebrum is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252535 for 10 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252535).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Caspr with ab252535 at 1/10000 dilution, followed by ready to use secondary antibody. Positive staining on mouse cerebrum is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252535 for 10 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252535).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Caspr with ab252535 at 1/10000 dilution, followed by ready to use secondary antibody. Positive staining on human cerebrum is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab252535 for 10 mins at room temperature.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252535).

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell line) cells labeling Caspr with ab252535 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in C6 cell line. The nuclear counter stain is DAPI (Blue). Tubulin is detected using ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    The negative controls are as follows:
    -ve control 1: ab252535 at 1/50 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (252535).

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling Caspr with ab252535 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. The nuclear counter stain is DAPI (Blue). Tubulin is detected using ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    The negative controls are as follows:
    -ve control 1: ab252535 at 1/50 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (252535).

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Caspr with ab252535 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counter stain is DAPI (Blue). Tubulin is detected using ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    The negative controls are as follows:
    -ve control 1: ab252535 at 1/50 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (252535).

  • Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)
    Anti-Caspr antibody [K65/35] - BSA and Azide free (ab255761)

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